Regulatory

Part:BBa_M1631:Design

Designed by: Oumou Diallo   Group: UtahState_BE5930_-_S11   (2013-04-12)


Mutated lacI 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1023
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Back translated and optimized to remove standard restriction sites


Source

LacI amino acid sequence with H2 binding-related amino acids, as discussed in www.sciencemag.org/cgi/content/321/5888/572/DC1 (GenBank: AAB98710.1)

References